FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_28.3510000005c098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_28.3510000005c098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59612
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGACGAGATCTGTCTCTTATACAC3450.578742535060055No Hit
AGAGGACGAGCTGTCTCTTATACACAT2360.39589344427296513No Hit
AGAGGACGAGATGCTGTCTCTTATACA650.10903844863450311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAACG455.037218E-721.448842
TAGGACG250.002956098221.448842
AGGACGA52150.021.448843
AGAGGAC51850.021.448841
AGTGGAC1300.021.448841
GCGGACG3750.021.448842
AGAACGA455.037218E-721.448843
GAGCACG404.384561E-621.448842
AGAGCAC404.384561E-621.448841
TGGACGA1300.021.448843
AGAGAAC455.037218E-721.448841
AGCACGA404.384561E-621.448843
GTGGACG1300.021.448842
GAGGACG51950.021.4281962
CGGACGA3800.021.1666163
AGCGGAC3800.021.1666161
AGCTTTT558.90941E-920.92701518
AAATTTT558.90941E-920.92701518
GATGTGG558.90941E-920.92701510
CCGTTTT558.90941E-920.92701518
Produced by FastQC (version 0.11.2)