FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_26.3510000005c048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_26.3510000005c048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71654
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC7100.9908728054260753No Hit
ACTGTCTCTGTCTCTTATACACATCTC5940.8289837273564631No Hit
ACTGTCTGTGTCTGTCTCTTATACACA970.1353727635582103No Hit
ACTGTCTGTCTCTTATACACATCTCCG820.1144388310492087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTG850.021.3980032
CAGTCTG659.458745E-1121.3980032
TGACTGT800.021.3980033
ACTGACT800.021.3980031
ACCGTCT751.8189894E-1221.3980031
CTGACTG800.021.3980032
ATTGTCT850.021.3980031
ACGGTCT1900.021.3980031
CTGTCTG63400.021.3473782
ACTGTCT64350.021.181861
GTCATTG303.9662363E-420.93510210
GTGTCGA4100.020.9351028
GTGTCAG4450.020.9351028
GAAATTT303.9662363E-420.93510217
AAATTTT601.0604708E-920.93510218
TGCGTTT303.9662363E-420.93510217
TTTCTTT303.9662363E-420.93510217
TGTCTTG1150.020.9351029
TGTCTTA1150.020.9351029
TGTCCGC1050.020.9351029
Produced by FastQC (version 0.11.2)