FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_25.3510000005c02e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_25.3510000005c02e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18421
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC570.30942945551273005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCG17200.021.2983443
ATGGTTC250.002998877521.2983442
CATGGTT250.002998877521.2983441
CTTGGTT16650.021.2343851
TTGGTTC16650.021.2343852
GTTGAGG506.765367E-820.9510889
GTTGAGA250.003301018220.9510889
CAGTTTT506.765367E-820.95108818
TTGCAGT250.003301018220.95108810
TTCGTTG18300.020.9510886
AATTTTT1000.020.95108819
TTATTTT250.003301018220.95108818
CGCTTTT354.3728585E-520.95108818
GAGTTTT354.3728585E-520.95108818
TTGGGGG506.765367E-820.95108810
TTGGGGA250.003301018220.95108810
TTGGGCG354.3728585E-520.95108810
TTGGGAG250.003301018220.95108810
GTCTTTT354.3728585E-520.95108818
CCTTTTT701.2732926E-1120.95108819
Produced by FastQC (version 0.11.2)