FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_24.3510000005c011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_24.3510000005c011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46390
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC3860.8320758784220738No Hit
GTGTAGTGTGGCTGTCTCTTATACACA3130.6747143780987281No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC1400.30178917870230654No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC1330.28669971976719116No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC1200.2586764388876913No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC1170.252209527915499No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA1140.24574261694330674No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC970.20909678810088378No Hit
GTGTAGTGTGGACCTGTCTCTTATACA920.19831860314723002No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC900.1940073291657685No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT660.14227204138823024No Hit
GTGTAGTGTGGATCCTGTCTCTTATAC620.1336494934253072No Hit
GTGTAGTGTGGAATCTGTCTCTTATAC600.12933821944384566No Hit
GTGTAGTGTGGATCTGTCTCTTATACA600.12933821944384566No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC560.12071567148092262No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC500.10778184953653806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGT40200.021.3362641
GGGTAGT506.060691E-821.3362641
CGTAGTG659.458745E-1121.3362642
GTTTAGT455.246475E-721.3362641
TTTAGTG455.246475E-721.3362642
TGTAGTG40200.021.3362642
GGTAGTG506.060691E-821.3362642
TGTCGTG2700.021.3362642
GTAGTGT41800.021.310743
ACTGTTT250.00338279920.94498417
GGATGTA405.348318E-620.94498410
GAGGCGG250.00338279920.94498411
GAGGTCG250.00338279920.94498411
TGCATTT250.00338279920.94498417
GCCTTTT456.27404E-720.94498418
CAGTTTT456.27404E-720.94498418
ATCTTTT250.00338279920.94498418
TAGTGTG43150.020.9449844
AAATTTT250.00338279920.94498418
AATTTTT1450.020.94498419
Produced by FastQC (version 0.11.2)