FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_22.3510000005bfdf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_22.3510000005bfdf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67837
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAACTACCCCTGTCTCTTATACA2090.3080914545159721No Hit
GTCAGAACTACCGCTGTCTCTTATACA1950.2874537494287778No Hit
GTCAGAACTACCTCTGTCTCTTATACA820.12087798693928091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAACT61850.021.2149283
TTAGAAC1100.021.2149262
TAGAACT1100.021.2149263
ACAGAAC608.949428E-1021.2149262
GACAGAA608.949428E-1021.2149261
GTCAAAA303.6226815E-421.2149261
GTCCGAA3150.021.2149261
CCAGAAC608.949428E-1021.2149262
TAAGAAC303.6226815E-421.2149262
GCCAGAA608.949428E-1021.2149261
TCAAAAC303.6226815E-421.2149262
TCAGAAC59900.021.2149262
AAGAACT303.6226815E-421.2149263
GTTAGAA1100.021.2149261
TCCGAAC3150.021.2149262
GTCAGAA59850.021.2149261
GGCAGAA751.8189894E-1221.2149261
CAAAACT303.6226815E-421.2149263
CCGAACT3150.021.2149263
ACCGGAC405.365515E-620.96460310
Produced by FastQC (version 0.11.2)