FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_21.3510000005bfb5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_21.3510000005bfb5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82509
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC1220.14786265740707075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTGA2150.021.3894751
CCTTGAA75150.021.3894752
TCTTGAA2150.021.3894752
CTTGAAC77350.021.3894753
TTTGAAC1700.021.3894733
CATTGAA950.021.3894732
ACATTGA950.021.3894731
ACCTTGA75100.021.3894731
CTAGAAC303.4360448E-421.3894733
ACCTAGA303.4360448E-421.3894731
CTTTGAA1700.021.3894732
CCTAGAA303.4360448E-421.3894732
CTTTCTC354.623182E-520.94845421
CTTATAC558.929419E-920.94845221
TGGTCTA250.003413718420.93575910
TTGTTTT701.4551915E-1120.93575918
TGGTTTA507.592098E-820.93575910
TGGTCGT354.644873E-520.93575910
TGGTCGG507.592098E-820.93575910
TGGTTCG250.003413718420.93575910
Produced by FastQC (version 0.11.2)