FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_2.3510000005bd5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_2.3510000005bd5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49544
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGCTGTCTCTTATA910.18367511706765702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGTG1150.021.2602461
GGTAATG354.0812818E-521.2602461
GGTCGTG2300.021.2602461
GGAAGTG303.551616E-421.2602441
GGTAGTG42600.021.2602441
GAGTGAG1600.021.2384853
GTCGTGA2300.021.2384852
TCGTGAG2300.021.2384853
GCAGTGA1150.021.2384852
GTAATGA354.113747E-521.2384852
CAGTGAG1150.021.2384853
GGAGTGA1600.021.2384852
GTAGTGA42700.021.2384832
TAGTGAG43050.021.1644843
CTACAGT250.003372355820.95959710
CTACACG303.911326E-420.95959710
TCCTTTT303.911326E-420.95959718
GGCATTT250.003372355820.95959717
CTATATC250.003372355820.95959710
GTGCATT250.003372355820.95959716
Produced by FastQC (version 0.11.2)