FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_19.3510000005bf72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_19.3510000005bf72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71782
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAGCGCATTGGGGGCTGTCTCTTA780.10866233874787551No Hit
TCAGAGCGCATTCTGTCTCTTATACAC740.10308991111977932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGC61800.021.2788413
CTGAGCG1650.021.278842
TCTGAGC1650.021.278841
CGAGCGC3200.021.278843
CCGAGCG3200.021.278842
TAGAGCG950.021.278842
AGCGCGC3200.021.278843
CAGCGCG3200.021.278842
TCCGAGC3200.021.278841
TCAGAGC61300.021.2614821
CAGAGCG61000.021.2613962
CGCATTC14450.020.9526167
GCATTAA3300.020.9526168
TAGAAGG250.00339356320.95261412
TCCTTTT405.3994154E-620.95261418
TTCATTT250.00339356320.95261417
GGGTTTT3000.020.95261418
CATTGTG1600.020.9526149
GCCTTTT900.020.95261418
GTCATTT354.605371E-520.95261417
Produced by FastQC (version 0.11.2)