FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_17.3510000005bf3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_17.3510000005bf3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36780
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTGCGTATGCTGTCTCTTATACAC2690.7313757476889614No Hit
AACGTGCGTATGGCTGTCTCTTATACA1130.3072321914083741No Hit
AACGTGCGTATGGGCTGTCTCTTATAC1100.29907558455682437No Hit
AACGTGCGTATGCTGTCTCTTCTACAC1060.28820010875475804No Hit
AACGTGCGTATCTGTCTCTTATACACA1020.27732463295269166No Hit
AACGTGCGTATGCCTGTCTCTTATACA830.22566612289287658No Hit
AACGTGCGTATGAGCTGTCTCTTATAC600.1631321370309951No Hit
AACGTGCGTATGGCCTGTCTCTTATAC540.1468189233278956No Hit
AACGTGCGTATGTCTGTCTCTTATACA500.13594344752582926No Hit
AACGTGCGTATGACCTGTCTCTTATAC400.1087547580206634No Hit
AACGTGCGTATGTGCTGTCTCTTATAC370.10059815116911365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC353.772062E-521.4518831
AACGTGC33850.021.4518831
TCGTGCG303.3187156E-421.4518832
AAGTGCG404.301919E-621.4518832
ATCGTGC303.3187156E-421.4518831
AAAGTGC404.301919E-621.4518831
AATGTGC607.4396667E-1021.4518831
ACGCGCG353.772062E-521.4518832
CGCGCGT353.772062E-521.4518833
TGTGCGT607.4396667E-1021.4518833
ACGTGCG33800.021.4518832
CGTGCGT34150.021.4204753
ATGGGTG303.926473E-420.9265310
TTGTTTT601.0168151E-920.9265318
TGGTTTT900.020.9265318
GTATGTG2150.020.926538
GTATGTC1100.020.926538
GTATGTA1550.020.926538
ATGGGGG1100.020.9265310
ATGGGCT303.926473E-420.9265310
Produced by FastQC (version 0.11.2)