FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_11.3510000005be73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_11.3510000005be73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74745
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCCTCAGCTGTCTCTTATACACA980.1311124489932437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACC1100.021.4824751
ATTGCCT1100.021.4824752
TTGCCTC1100.021.4824753
AATTGCC1100.021.4824751
ATGTGCC1000.021.4824731
AAGTGCC67950.021.4824731
AAATGCC250.00293636421.4824731
AAGTCCC800.021.4824731
GTGCCTC69000.021.451343
AGTGCCT68250.021.3408322
CTTCATT250.0034240520.92168816
TCCTTTT559.027644E-920.92168818
TTCATTT250.0034240520.92168817
TGCATTT250.0034240520.92168817
GTGTTTA354.6613914E-520.92168811
CCGTGTT250.0034240520.92168816
CAGTCTT601.0713848E-920.9216889
CAGTCTA701.4551915E-1120.9216889
GTTTGGT354.6613914E-520.92168811
CAGTTTG1750.020.9216889
Produced by FastQC (version 0.11.2)