FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_94.3510000005bcd2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_94.3510000005bcd2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22501
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA1300.5777520999066709No Hit
TACTGCAAGGAGGCTGTCTCTTATACA380.16888138304964223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAAG505.3916665E-821.4510253
ACTGCAA20800.021.4510252
TTGCAAG250.002896634621.4510253
TATTGCA250.002896634621.4510251
ATTGCAA250.002896634621.4510252
TACTGCA20800.021.4510251
CTGCAAG20950.021.4510253
TACTACA505.3916665E-821.4510251
ACTACAA505.3916665E-821.4510252
GAGCGGG354.4695826E-520.92666810
CAGTTTT354.4695826E-520.92666818
ACGTTTT354.4695826E-520.92666818
GAGTCGG354.4695826E-520.92666810
GGAGGTA354.4695826E-520.9266689
AAGGAGT5150.020.9266687
CAAGGAG22300.020.9266686
GGAGCGC354.4695826E-520.9266689
AGGAGTT1250.020.9266688
GGAGTCG354.4695826E-520.9266689
AGGAGCC701.2732926E-1120.9266688
Produced by FastQC (version 0.11.2)