FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_93.3510000005bcb8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_93.3510000005bcb8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15462
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGACGCTAGGGCTGTCTCTTATA200.1293493726555426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC800.021.2360672
ATCTAGA800.021.2360671
CTAGACG800.021.2360673
CTCGACG13750.021.2360653
ATCTCGA13350.021.2360651
AGCTCGA250.003028330221.2360651
TTCGACG455.01077E-721.2360653
TCTCGAC13350.021.2360652
GCTCGAC250.003028330221.2360652
ATTTCGA455.01077E-721.2360651
TTTCGAC455.01077E-721.2360652
ACGCTAT3950.020.9611667
ACGCTAC3100.020.9611667
TCGACGC14450.020.9611664
GCTAGTA404.9430528E-620.9611669
AATTTTT850.020.96116619
GCTAGGT404.9430528E-620.9611669
TAGTTTT404.9430528E-620.96116618
CGCTAGG2450.020.9611668
CGCTAGC800.020.9611668
Produced by FastQC (version 0.11.2)