FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_88.3510000005bc0b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_88.3510000005bc0b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12763
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC1551.2144480137898614No Hit
GTTGAGCTTGACCTGTCTCTTCTACAC310.24288960275797225No Hit
GTTGAGCTTGACGCTGTCTCTTATACA290.227219305805845No Hit
GTTGAGCTTGACGGCTGTCTCTTATAC240.18804356342552692No Hit
GTTGAGCTTGACCCTGTCTCTTATACA210.16453811799733603No Hit
GTTGAGCTTGACTCTGTCTCTTATACA170.13319752409308155No Hit
GTTGAGCTTGACGGGGGGGGTTTTTTT140.1096920786648907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAGC9900.021.2868541
TTGAGCT9900.021.2868542
TGCGCTT1200.021.2868523
TGGAGCT505.3554686E-821.2868522
TTGCGCT1200.021.2868522
GTGGAGC505.3554686E-821.2868521
GGAGCTT505.3554686E-821.2868523
GTTGCGC1200.021.2868521
TGAGCTT10550.021.1859663
TGACTGC354.2344203E-520.9529449
TGACCCG354.2344203E-520.9529449
GACGGTG354.2344203E-520.95294410
TTGACCA354.2344203E-520.9529448
TTGACAA701.0913936E-1120.9529448
TCTTTTT354.2344203E-520.95294419
TGACGTC354.2344203E-520.9529449
TGACGCG701.0913936E-1120.9529449
GGCATTT250.003244233820.95294217
AGCTTGA11450.020.9529425
AAATTTT250.003244233820.95294218
Produced by FastQC (version 0.11.2)