FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_85.3510000005bbb9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_85.3510000005bbb9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17043
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA750.44006336912515404No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC320.1877603708267324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTA14850.021.3233552
CTCTCGT900.021.3233551
CTCTAGT14850.021.3233551
TCTCGTA900.021.3233552
CTCGTAC900.021.3233553
GCTAGTA250.002968896621.3233532
CGCTAGT250.002968896621.3233531
CTAGTAC15400.021.3233533
CACTAGT250.002968896621.3233531
ACTAGTA250.002968896621.3233532
TTGTTTT506.701521E-820.9470618
TGCGTTT303.7822832E-420.9470617
GCGATTT250.003294396720.9470617
GTACCGC506.701521E-820.947066
GTACCGA16300.020.947066
TTATTTT354.3538646E-520.9470618
TTTTCTG250.003294396720.9470621
AGGGTTT354.3538646E-520.9470617
GGATTTT303.7822832E-420.9470618
GTCTGTC608.949428E-1020.9470620
Produced by FastQC (version 0.11.2)