FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_80.3510000005bb0c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_80.3510000005bb0c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20130
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC1340.6656731246895181No Hit
GGATAGTTAGCCTGTCTCTTATACACA480.23845007451564829No Hit
GGATAGTTAGCCCTGTCTCTTCTACAC440.2185792349726776No Hit
GGATAGTTAGCCGCTGTCTCTTATACA280.13909587680079483No Hit
GGATAGTTAGCCGGGGGGGGTTTTTTT240.11922503725782414No Hit
GGATAGTTAGCTGTCTCTTATACACAT240.11922503725782414No Hit
GGATAGTTAGCCCCTGTCTCTTATACA210.10432190760059612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTT800.021.4122142
GGTTAGT950.021.4122141
GTTAGTT950.021.4122142
CTAGTTA800.021.4122143
GGCTAGT800.021.4122141
TTAGTTA950.021.4122143
GGATAGT16450.021.4122121
ATAGTTA16450.021.4122123
ATCGTTA1300.021.4122123
GATAGTT16450.021.4122122
AGCCCGG609.331416E-1020.9328379
AGCCCGA250.003328392320.9328379
AGCCTGA506.9090675E-820.9328379
TTGTTTT609.331416E-1020.93283718
AGCCTAC250.003328392320.9328379
AGCCAGA303.8334928E-420.9328379
AGCCACG506.9090675E-820.9328379
AGCCAAT303.8334928E-420.9328379
AGCCAAG609.331416E-1020.9328379
GCGATTT250.003328392320.93283717
Produced by FastQC (version 0.11.2)