FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_8.3510000005b256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_8.3510000005b256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC711.79883455789207No Hit
ACAGTGCTCATGCTGTCTCTTCTACAC380.9627565239422345No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC130.32936407398023815No Hit
ACCGTGCTCATGCTGTCTCTTCTACAC110.2786926779832784No Hit
ACAGTGCTCATGCCCTGTCTCTTATAC80.20268558398783887No Hit
ACAGTGCTCATGGCTGTCTCTTATACA80.20268558398783887No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC70.177349885989359No Hit
ACAGTGCTCATGCGCTGTCTCTTATAC70.177349885989359No Hit
ACCGTGCTCATGCTGTCTCTTATACAC70.177349885989359No Hit
ACAGTGCTCCTGTCTCTTATACACATC60.15201418799087915No Hit
ACAGTGCTCATGGGGGGGCTGTCTCTT50.12667848999239928No Hit
ACAGTGCTCATGGGGCTGTCTCTTATA50.12667848999239928No Hit
ACAGTGCTCATGGGGGAAGGTTTTTTT50.12667848999239928No Hit
ACAGTGCTCATGGGGGGAGGTTTTTTT50.12667848999239928No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC50.12667848999239928No Hit
ACAGTGCTCATGCCTGTCTCTTATACA50.12667848999239928No Hit
ACAGTGCTCATGGCGGGTGGTTTTTTT40.10134279199391943No Hit
ACAGTGCTCATGGGGGCCTGTCTCTTA40.10134279199391943No Hit
ACAGTGCTCATGGGTGGGAGTTTTTTT40.10134279199391943No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC40.10134279199391943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGC3250.021.01
GGGTTTT353.275441E-521.018
CCGTGCT453.6797974E-721.02
TTTTTTT2150.021.021
TGTTTTT250.002820193521.019
GTTTTTT800.021.020
GGTTTTT403.4876957E-621.019
ACCGTGC453.6797974E-721.01
GTCTGTC250.002820193521.020
AGTGCTC3250.021.03
TGCTCAT3750.021.05
TCATGGT403.4876957E-621.08
TCATGGG604.0563464E-1021.08
TCATGGC353.275441E-521.08
TCATGCT453.6797974E-721.08
TCATGCG503.8440703E-821.08
TCATGAG303.050626E-421.08
TCATGAC250.002820193521.08
GATTTTT353.275441E-521.019
CGTGCTC453.6797974E-721.03
Produced by FastQC (version 0.11.2)