FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_79.3510000005bae1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_79.3510000005bae1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18621
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAAAAAAAAAAAAAAAAA550.29536544761291017No Hit
CAGACATCGCAACCTGTCTCTTATACA220.11814617904516406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCG900.021.1463413
GACATCG16700.021.1463413
AGAAATC900.021.1463412
CAGACAT16700.021.1463411
CAGAAAT900.021.1463411
AGACATC16650.021.1463412
CAGCCAT557.044946E-921.146341
AGCCATC557.044946E-921.146342
GCCATCG557.044946E-921.146343
TGGTTTT455.7841316E-720.97580718
CAATGTG303.7659795E-420.97580710
GTGTTTT651.03682396E-1020.97580718
GGGTTTT900.020.97580718
CAATGGG405.0045273E-620.97580710
GCAAGTT651.03682396E-1020.9758079
GCAAGTG800.020.9758079
GCAAGGG651.03682396E-1020.9758079
GCAAGGC800.020.9758079
GCAAGCG405.0045273E-620.9758079
GCAAGAT405.0045273E-620.9758079
Produced by FastQC (version 0.11.2)