FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_73.3510000005ba27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_73.3510000005ba27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8291
Sequences flagged as poor quality0
Sequence length27
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC450.5427572066095766No Hit
CTTGGTTCGTTGGCTGTCTCTTATACA140.1688577976118683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCG7350.021.220863
CTTGGTT7150.021.220861
TTGGTTC7150.021.220862
TCGTTGC1750.020.9636387
GTTGAGC250.003138684420.9636369
GTTGAAG353.986255E-520.9636369
TTCGTTG8200.020.9636366
AATTTTT404.486483E-620.96363619
GGTTCGT7900.020.9636364
GAGTTTT505.6490535E-820.96363618
ACTTTTT657.8216544E-1120.96363619
AGAGTTT250.003138684420.96363617
CCTTTTT250.003138684420.96363619
TCGTTGT1450.020.9636367
TCGTTGG3150.020.9636367
TCGTTGA1900.020.9636367
GATTTTT607.039489E-1020.96363619
TTGGCTG303.537863E-420.96363610
GTTGGTA303.537863E-420.9636369
GTTGGGT303.537863E-420.9636369
Produced by FastQC (version 0.11.2)