FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_72.3510000005ba1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_72.3510000005ba1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12699
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC1481.165446098117962No Hit
GTGTAGTGTGGCTGTCTCTTATACACA770.6063469564532641No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC580.4567288762894716No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC530.41735569729899996No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC440.34648397511615087No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC430.33860933931805653No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC400.31498543192377354No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC350.2756122529333019No Hit
GTGTAGTGTGGACCTGTCTCTTATACA330.25986298133711316No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA320.25198834553901883No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC270.21261516654854712No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC190.1496180801637924No Hit
GTGTAGTGTGGATCCTGTCTCTTATAC190.1496180801637924No Hit
GTGTAGTGTGGATACTGTCTCTTATAC180.14174344436569808No Hit
GTGTAGTGTGGATCTGTCTCTTATACA170.13386880856760375No Hit
GTGTAGTGTGGAAAAAAAAAAAAAAAA170.13386880856760375No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT160.12599417276950942No Hit
GTGTAGTGTGGATTCTGTCTCTTATAC160.12599417276950942No Hit
GTGTAGTGTGGACACTGTCTCTTATAC150.11811953697141507No Hit
GTGTAGTGTGGAGGGCTGTCTCTTATA140.11024490117332074No Hit
GTGTAGTGTGGACTCTGTCTCTTATAC130.1023702653752264No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGT10550.021.3629061
TGTAGTG10550.021.3629062
GTGTCGT900.021.3629041
AGTAGTG353.6357953E-521.3629042
GTCGTGT900.021.3629043
GAGTAGT353.6357953E-521.3629041
TGTCGTG900.021.3629042
GTAGTGT11150.021.3629043
TGATTTT354.250074E-520.94071218
TATTTTT354.250074E-520.94071219
TGGATGT354.250074E-520.9407129
TGTGGAG5100.020.9407127
GTGGATG1250.020.9407128
GTGTGGC354.250074E-520.9407126
GTGTGGA12200.020.9407126
TGGTTTT303.7156383E-420.9407118
GTGTTTT455.573038E-720.9407118
GGGTTTT557.2996045E-920.9407118
TAGTGTG11550.020.940714
AATTTTT404.8660822E-620.9407119
Produced by FastQC (version 0.11.2)