FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_71.3510000005b9e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_71.3510000005b9e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21647
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA1730.7991869543123759No Hit
ATCTGCTATCCTGTCTCTTATACACAT500.2309788885295884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCTA19500.021.426542
GCTGCTA250.002912165121.426542
CTGCTAT19900.021.426543
ATCTGCT19500.021.426541
AGCTGCT250.002912165121.426541
TATGCTA303.2823533E-421.4265382
ATCTACT303.2823533E-421.4265381
TCTACTA303.2823533E-421.4265382
ATGCTAT303.2823533E-421.4265383
ATATGCT303.2823533E-421.4265381
ATTTGCT353.711359E-521.4265381
TTTGCTA353.711359E-521.4265382
CTACTAT303.2823533E-421.4265383
TTGCTAT353.711359E-521.4265383
ACGTTTT455.9986814E-720.93055718
TTATTTT455.9986814E-720.93055718
CGCTTTT405.1632232E-620.93055718
TGTTTTT1900.020.93055719
GGATTTT405.1632232E-620.93055718
ACTTTTT800.020.93055719
Produced by FastQC (version 0.11.2)