FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_7.3510000005b249.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_7.3510000005b249.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60862
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC1940.3187539022707108No Hit
CTTCAGCAATGCGGGGGGCTGTCTCTT660.10844204922611811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC701.2732926E-1121.2550011
ACAGCAA701.2732926E-1121.2550013
TACAGCA701.2732926E-1121.2550012
CCAGCAA1100.021.2550013
CTCCAGC1100.021.2550011
TTCAGCA50850.021.2550012
GTCAGCA557.4505806E-921.2550012
CTTCAGC50800.021.2551
TTCCGCA3850.021.2552
TTCTGCA455.503025E-721.2552
CATCAGC303.570762E-421.2551
ATCAGCA303.570762E-421.2552
TCAGCAA51900.021.2553
TCTGCAA455.503025E-721.2553
CTCAGCA250.003117098221.2552
CCTCAGC250.003117098221.2551
TCCGCAA3850.021.2553
CTTCCGC3850.021.2551
CTTCTGC455.503025E-721.2551
CAATGCC10650.020.9580967
Produced by FastQC (version 0.11.2)