FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_67.3510000005b978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_67.3510000005b978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17172
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAGCGCATTCTGTCTCTTATACAC360.20964360587002098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAGCG15100.021.2662722
AGAGCGC15450.021.2662723
TCAGAGC15100.021.2662721
CGAGCGC455.009788E-721.2662723
CCGAGCG455.009788E-721.2662722
AGCGCGC709.094947E-1221.2662723
CAGCGCG709.094947E-1221.2662722
TCCGAGC455.009788E-721.2662721
AATTTTT1100.020.9562719
TGTTTTT1100.020.9562719
TATTTTT557.719791E-920.9562719
GCATTGA1050.020.956278
GCATTCA1050.020.956278
GCATTAT1100.020.956278
TGGTTTT354.3420463E-520.95626818
GTGTTTT354.3420463E-520.95626818
GGGTTTT850.020.95626818
CATTGTC354.3420463E-520.9562689
CATTGTA303.772966E-420.9562689
GCCTTTT303.772966E-420.95626818
Produced by FastQC (version 0.11.2)