FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_64.3510000005b91b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_64.3510000005b91b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17238
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC2431.4096762965541247No Hit
GAACACCATAGCGGCTGTCTCTTATAC1120.6497273465599257No Hit
GAACACCATAGCCTGTCTCTTCTACAC890.5163011950342267No Hit
GAACACCATAGCGCCTGTCTCTTATAC750.43508527671423597No Hit
GAACACCATAGCAGCTGTCTCTTATAC570.33066481030281936No Hit
GAACACCATAGCCCCTGTCTCTTATAC540.3132613992342499No Hit
GAACACCATAGCACCTGTCTCTTATAC430.24944889198282866No Hit
GAACACCATAGCTGCTGTCTCTTATAC430.24944889198282866No Hit
GAACACCATAGCGTCTGTCTCTTATAC410.2378466179371157No Hit
GAACACCATAGCGACTGTCTCTTATAC390.22624434389140274No Hit
GAACACCATAGCTGTCTCTTATACACA380.22044320686854624No Hit
GAACACCATAGCATCTGTCTCTTATAC370.21464206984568973No Hit
GAACACCATAGCCCTGTCTCTTATACA340.19723865877712032No Hit
GAACACCATAGCCGCTGTCTCTTATAC340.19723865877712032No Hit
GAACACCATAGCTCCTGTCTCTTATAC260.1508295625942685No Hit
GAACACCATAGCTTCTGTCTCTTATAC230.13342615152569903No Hit
GAACACCATAGCCTCTGTCTCTTATAC230.13342615152569903No Hit
GAACACCATAGCAACTGTCTCTTATAC210.12182387747998608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCAT14950.021.2654883
AACCCCA709.094947E-1221.2654882
TACACCA404.4046446E-621.2654882
ACCCCAT709.094947E-1221.2654883
ACCACCA1050.021.2654862
CCACCAT1050.021.2654863
GAACACC14550.021.2654861
GACCACC1050.021.2654861
AACACCA14550.021.2654862
GGTTTTT1450.020.95639819
ATAGCAG1450.020.9563988
TAGCGGC557.723429E-920.9563969
TAGCGGA354.3428023E-520.9563969
TAGCGCT303.7734368E-420.9563969
TAGCGCC557.723429E-920.9563969
TAGCGCA303.7734368E-420.9563969
TAGCGAG455.782513E-720.9563969
TAGCGAC250.00328746220.9563969
GGGTTTT701.2732926E-1120.95639618
AATTTTT506.681876E-820.95639619
Produced by FastQC (version 0.11.2)