FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_61.3510000005b8a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_61.3510000005b8a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14013
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTATGGTCTGTCTCTTATACACA800.5708984514379505No Hit
GACTCTATGGTGGCTGTCTCTTATACA600.42817383857846286No Hit
GACTCTATGGTGTCTGTCTCTTATACA380.27117676443302646No Hit
GACTCTATGGTGCCTGTCTCTTATACA310.2212231499322058No Hit
GACTCTATGGTGACTGTCTCTTATACA180.12845215157353884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAT12050.021.1949462
CTCTATG12150.021.1949463
GACTCTA12050.021.1949461
CTATATG1000.021.1949443
GACTATA1000.021.1949441
ACTATAT1000.021.1949442
GCGTTTT659.640644E-1120.9678618
GGTGGGG659.640644E-1120.967869
GGTGGCG659.640644E-1120.967869
TGGTGCT659.640644E-1120.967868
TGGTTTT506.4195774E-820.96785718
GGGTTTT506.4195774E-820.96785718
GGTGAGT250.003246754420.9678579
GGTGAGA250.003246754420.9678579
AGCTTTT303.71092E-420.96785718
TGCGTTT303.71092E-420.96785717
TATATGG1000.020.9678574
ACGTTTT303.71092E-420.96785718
TGTTTTT1050.020.96785719
ATGGTGT3450.020.9678577
Produced by FastQC (version 0.11.2)