FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_60.3510000005b886.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_60.3510000005b886.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18621
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC1430.7679501637935664No Hit
GAATCCTTACGCCTGTCTCTTCTACAC360.1933301111648139No Hit
GAATCCTTACGCTGTCTCTTATACACA300.16110842597067826No Hit
GAATCCTTACGCCCTGTCTCTTATACA250.13425702164223188No Hit
GAATCCTTACGCGCTGTCTCTTATACA220.11814617904516406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTT16250.021.2950822
CTCCTTA709.094947E-1221.2950823
ACTCCTT709.094947E-1221.2950822
GTATCCT454.9922164E-721.2950821
TTCCTTA250.0030028121.2950823
TATCCTT454.9922164E-721.2950822
GAATCCT16250.021.2950821
GATTCCT250.0030028121.2950821
AATACTT454.9922164E-721.2950822
GAATACT454.9922164E-721.2950821
ATACTTA454.9922164E-721.2950823
ATTCCTT250.0030028121.2950822
GACTCCT709.094947E-1221.2950821
ATCCTTA16700.021.2950823
GTGTTTT701.2732926E-1120.95161418
ACGCCTG354.3753418E-520.9516149
ACGCCGT303.7954526E-420.9516149
ACGCCGC354.3753418E-520.9516149
ACGCTGC354.3753418E-520.9516149
ACGCCAA303.7954526E-420.9516149
Produced by FastQC (version 0.11.2)