FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_58.3510000005b843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_58.3510000005b843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17370
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC1881.0823258491652272No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC610.3511801957397812No Hit
ACGTCTCTGCCTGTCTCTTATACACAT570.3281519861830743No Hit
ACGTCTCTGCCTGTCTCTTATCCACAT250.14392630972941853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTC15250.021.322581
ACGTATC1350.021.322581
GTATCTG1350.021.2602353
CGTATCT1350.021.2602352
CGTCTCT15300.021.2602352
GTCTCTG15500.021.1916543
TTGTTTT455.795282E-720.95389218
CTCTGCA15850.020.9538925
ATGGGGG455.795282E-720.95389211
TGCATGT1450.020.9538928
TGCATGA1450.020.9538928
TGTTTTT1700.020.95389219
GTATTTT455.795282E-720.95389218
TAGTTTT455.795282E-720.95389218
ACTTTTT850.020.95389219
GCATGGG1050.020.9538929
TCTCTGC15850.020.9538924
CTGCATT3600.020.9538927
CTGCATA3050.020.9538927
AGATTTT455.795282E-720.95389218
Produced by FastQC (version 0.11.2)