FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_55.3510000005b7e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_55.3510000005b7e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21253
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC950.446995718251541No Hit
CTTCAGCAATGCCTGTCTCTTCTACAC300.14115654260574978No Hit
CTTCAGCAATGCGGCTGTCTCTTATAC230.10822001599774149No Hit
CTTCAGCAATGCGGGGGGGGTTTTTTT220.10351479791088317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGCA1250.021.2577572
TCAGCAA18100.021.2577573
GCAGCAA455.1514326E-721.2577573
TCCGCAA1250.021.2577573
CTGCAGC455.1514326E-721.2577571
TGCAGCA455.1514326E-721.2577572
CTTCCGC1250.021.2577571
CTTCAGC17700.021.2577551
CCAGCAA505.9006197E-821.2577553
TCGGCAA250.003048201821.2577553
CTCCAGC505.9006197E-821.2577551
TCCAGCA505.9006197E-821.2577552
CTTCGGC250.003048201821.2577551
TTCAGCA17700.021.2577552
TTCGGCA250.003048201821.2577552
TCCTTTT303.8129423E-420.95764718
GTGTTTT751.8189894E-1220.95764718
GGGTTTT900.020.95764718
CAATGCG8350.020.9576477
CAATGCC4350.020.9576477
Produced by FastQC (version 0.11.2)