FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_54.3510000005b7ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_54.3510000005b7ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18618
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA2101.1279407025459234No Hit
CTGATCGTAGCACCTGTCTCTTATACA730.3920936727897733No Hit
CTGATCGTAGCAGCTGTCTCTTATACA420.2255881405091847No Hit
CTGATCGTAGCAGGGGCTGTCTCTTAT250.13427865506499087No Hit
CTGATCGTAGCAAAAAAAAAAAAAAAA230.12353636265979161No Hit
CTGATCGTAGCATCTGTCTCTTATACA220.11816521645719197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA850.021.1463433
TGCTCGT850.021.1463432
CTGCTCG850.021.1463431
GAACGTA250.00312841621.1463413
TGATCGT16850.021.1463412
GATCGTA17100.021.1463413
CTGATCG16850.021.1463411
CTGAACG250.00312841621.1463411
TGAACGT250.00312841621.1463412
TTGTTTT405.0045273E-620.97580718
GGGTTTT1300.020.97580718
GCAAGGG303.7659795E-420.97580710
AATTTTT850.020.97580719
TTTCTGT405.0045273E-620.97580721
GTTCTGT303.7659795E-420.97580720
TGATTTT303.7659795E-420.97580718
GCAGGCG405.0045273E-620.97580710
GCAGGCC405.0045273E-620.97580710
GGATTTT608.967618E-1020.97580718
CAATTTT303.7659795E-420.97580718
Produced by FastQC (version 0.11.2)