FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_52.3510000005b794.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_52.3510000005b794.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14344
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTCGATACCTGTCTCTTATACAC2571.7916899051868378No Hit
AGACGTCGATACCTGTCTCTTCTACAC750.5228667038482989No Hit
AGACGTCGATACCCTGTCTCTTATACA240.16731734523145567No Hit
AGACGTCGATACGCTGTCTCTTATACA160.11154489682097045No Hit
AGACGTCGATACGGCTGTCTCTTATAC160.11154489682097045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA12550.021.3131683
AGCCGTC850.021.3131681
GTCGTCG303.323878E-421.3131662
AGACGTC12350.021.3131661
AGTCGTC303.323878E-421.3131661
TCGTCGA303.323878E-421.3131663
GACGTCG12400.021.2272262
TATACAC404.7920985E-621.01403621
CTTTTTT3000.021.01403420
CGATACG5800.020.9405617
GATACTT800.020.9405618
GATACTG1450.020.9405618
GATACGG2250.020.9405618
ATACGGT404.9418704E-620.9405619
GGCTTTT404.9418704E-620.94056118
TCGTTTT404.9418704E-620.94056118
ATACCTG800.020.9405619
ATACTGT404.9418704E-620.9405619
TTGTTTT455.6819044E-720.9405618
GGTTGTT250.003274813820.9405616
Produced by FastQC (version 0.11.2)