FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_50.3510000005b751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_50.3510000005b751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14013
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGCTGTCTCTTATA190.13558838221651323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGAG303.4477553E-421.1949463
GTCGTGA850.021.1949462
GAAGTGA303.4477553E-421.1949462
GGGAGTG303.4477553E-421.1949461
AAGTGAG303.4477553E-421.1949463
GGAGTGA303.4477553E-421.1949462
GGTCGTG850.021.1949461
TAGTGAG11950.021.1949443
GCAGTGA250.003048421.1949442
GGTAGTG11900.021.1949441
GGCAGTG250.003048421.1949441
CAGTGAG250.003048421.1949443
GTAGTGA11950.021.1949442
GCTAGAG659.640644E-1120.967869
AGCTATA659.640644E-1120.967868
AGCTAGC1300.020.967868
GAGCTAA2550.020.967867
CAGTTTT250.003246754420.96785718
GCTAGTT250.003246754420.9678579
GCTAGTG506.4195774E-820.9678579
Produced by FastQC (version 0.11.2)