FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_47.3510000005b6e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_47.3510000005b6e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63101
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGACTGGAGGCTGTCTCTTATAC1260.19967987829035988No Hit
TAGCAGACTGGAGCTGTCTCTTATACA660.10459422196161709No Hit
TAGCAGACTGGAGCCTGTCTCTTATAC660.10459422196161709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGAC850.021.3489512
TCAGACT850.021.3489513
TAACAGA250.003039441521.3489511
TATCAGA850.021.3489511
AACAGAC250.003039441521.3489512
TAGAAGA303.4676553E-421.348951
AGAAGAC303.4676553E-421.348952
TAGCCGA3900.021.348951
TAGCTGA303.4676553E-421.348951
AGCCGAC3900.021.348952
GCCGACT3900.021.348953
AGCTGAC303.4676553E-421.348952
TAGCGGA353.9745413E-521.3489481
TTGCAGA701.0913936E-1121.3489481
TGCAGAC701.0913936E-1121.3489482
GCAGACT55900.021.3298533
TAGCAGA55250.021.329631
AGCAGAC55250.021.329632
GGAGATC250.003398156520.9429510
GATGGGG250.003398156520.9429511
Produced by FastQC (version 0.11.2)