FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_46.3510000005b6cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_46.3510000005b6cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90141
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA3550.39382744810907355No Hit
TACTGCAAGGAGGCTGTCTCTTATACA1130.12535915953894455No Hit
TACTGCAAGGAGGGGGGGCTGTCTCTT1070.11870292097935456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCA800.021.4505141
TCTGCAA800.021.4505142
CTACAAG850.021.4505123
ACTGCAA83300.021.4505122
TTGCAAG1350.021.4505123
CTCCAAG556.8121153E-921.4505123
ACTCCAA556.8121153E-921.4505122
TAATGCA659.276846E-1121.4505121
TATTGCA1350.021.4505121
ATTGCAA1350.021.4505122
TACTGCA83450.021.4505121
ATGCAAG659.276846E-1121.4505123
AATGCAA659.276846E-1121.4505122
TACTCCA556.8121153E-921.4505121
TACTACA850.021.4505121
ACTACAA850.021.4505122
CTGCAAG84150.021.450513
AGGGGTG303.9879448E-420.92674811
TGGTTTT850.020.92674818
GAGGTGA456.465543E-720.92674810
Produced by FastQC (version 0.11.2)