FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_44.3510000005b688.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_44.3510000005b688.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62308
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACA2000.021.2489831
AGCCACT56000.021.2489833
TTCGCCA2450.021.2489831
TAAGCCA455.5225246E-721.2489831
CGCCACT2450.021.2489833
GAGCCAC701.2732926E-1121.2489832
TCGCCAC2450.021.2489832
AAGCCAC455.5225246E-721.2489832
AGACACT2000.021.2489833
TTAGCCA54700.021.2489831
TGAGCCA701.2732926E-1121.2489831
TAGACAC2000.021.2489832
TAGCCAC54750.021.2489812
TTGCCAC751.8189894E-1221.2489812
CTCTGGG11750.020.959078
CTCTGCC2600.020.959078
CTCTGAC2600.020.959078
CTCTGAA2350.020.959078
GTCATTT456.305381E-720.9590717
ATCTTTT354.5819448E-520.9590718
Produced by FastQC (version 0.11.2)