FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_43.3510000005b67b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_43.3510000005b67b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207316
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGGATGCTGTCTCTTATACACA18850.9092400007717686No Hit
CTAGCTGGATGTCTGTCTCTTATACAC4300.20741283837233981No Hit
CTAGCTGGATGTGGGCTGTCTCTTATA2740.13216539003260722No Hit
CTAGCTGGATCTGTCTCTTATACACAT2620.12637712477570473No Hit
CTAGCTGGATGTGGGGGCTGTCTCTTA2570.12396534758532868No Hit
CTAGCTGGATGTGGGGCTGTCTCTTAT2220.10708290725269637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA6550.021.1665063
GAGCTGG2450.021.1665042
AGCTGGA187150.021.160853
CTAGCTG182550.021.1549111
TAGCTGG182600.021.143322
CTAGATG5750.020.9824471
GGAGATT405.4365537E-620.97250616
CTTTTCT405.4365537E-620.97250620
TTCATTT405.4365537E-620.97250617
TGTTGCA800.020.97250610
GCCTTTT1600.020.97250618
GTCATTT405.4365537E-620.97250617
CGCCTTT405.4365537E-620.97250617
CCAGTTT405.4365537E-620.97250617
AGGATTT800.020.97250617
TGTCTGA405.4365537E-620.97250610
TCGGTTT800.020.97250617
GTCCGTG405.4365537E-620.97250611
TGTTCTA405.4365537E-620.97250610
ACCGTTT405.4365537E-620.97250617
Produced by FastQC (version 0.11.2)