FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_42.3510000005b652.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_42.3510000005b652.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70798
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCAGA1150.021.310571
TCAGAGA1150.021.310573
TTCAGAG1150.021.310572
CAAGAGA506.257869E-821.3105683
CCGGAGA557.2795956E-921.3105683
TCCCAGA506.257869E-821.3105681
ACAGAGA455.3920485E-721.3105683
TACAGAG455.3920485E-721.3105682
TTCAAGA506.257869E-821.3105681
TTCCGGA557.2795956E-921.3105681
TTACAGA455.3920485E-721.3105681
TCCAGAG61150.021.3105682
TTCCCGA3800.021.3105681
TCCGGAG557.2795956E-921.3105682
TTCCAGA61150.021.3105681
CCCGAGA3800.021.3105683
TACCAGA455.3920485E-721.3105681
ACCAGAG455.3920485E-721.3105682
CCAGAGA62800.021.29363
TCCCGAG3850.021.0338082
Produced by FastQC (version 0.11.2)