FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_39.3510000005b5f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_39.3510000005b5f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37175
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA780.20981842636180226No Hit
ACTTCGTCAGCTGTCTCTTATACACAT520.1398789509078682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGT3100.021.2732261
ATTTCGT303.5136228E-421.2732261
ACATCGT404.6297682E-621.2732241
ACCTCGT404.6297682E-621.2732241
CTTAGTC3100.021.2442042
TTAGTCA3100.021.2442043
TTTCGTC303.5463093E-421.2442042
TTCGTCA31350.021.2442023
GTCGTCA900.021.2442023
CCTCGTC404.685162E-621.2442022
CTTCGTC31050.021.2442022
CGTCGTC900.021.2442022
ATCGTCA404.685162E-621.2442023
CTCGTCA404.685162E-621.2442023
CATCGTC404.685162E-621.2442022
ACTTCGT31050.021.2389681
CAGCTCG2450.020.9582798
CAGCTAA1550.020.9582798
AGCTTTG651.1823431E-1020.9582799
AGCTTGG751.8189894E-1220.9582799
Produced by FastQC (version 0.11.2)