FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_37.3510000005b5a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_37.3510000005b5a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35862
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA1850.5158663766661089No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC540.15057721264848586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT31000.021.2545991
TTTAGTA506.210939E-821.2545992
CACTAGT250.003092401221.2545991
ACTAGTA250.003092401221.2545992
TTAGTAC506.210939E-821.2545993
TCTAGTA31050.021.2545972
GCTAGTA608.3309715E-1021.2545972
CTCTCGT1850.021.2545971
CGCTAGT608.3309715E-1021.2545971
TCTCGTA1850.021.2545972
CTAGTAC32450.021.2545973
CCTAGTA557.192284E-921.2545972
CTCGTAC1850.021.2545973
CCCTAGT557.192284E-921.2545971
TTGTTTT751.8189894E-1220.9581618
GTACCGA34750.020.958166
GGTTTTT3000.020.9581619
CGAGGTG751.8189894E-1220.9581610
ACTTTTT751.8189894E-1220.9581619
TACCGAT6850.020.958167
Produced by FastQC (version 0.11.2)