FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_35.3510000005b56f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_35.3510000005b56f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185544
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA19201.0347949812443409No Hit
CACAGAAGGACTGGGGGCTGTCTCTTA1930.1040184538438322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAAAG1500.021.1610012
ACAGAAG166750.021.1610012
CACAAAA1500.021.1610011
CAAAGAA609.567884E-1021.1610011
CAGAAGG169400.021.1610013
CACAGAA166550.021.1610011
AAAGAAG609.567884E-1021.1610012
CAAAAGG1500.021.1610013
CACACAA558.112693E-921.1611
CACAAGG558.112693E-921.1613
ACCGAAG8650.021.1612
ACACAAG558.112693E-921.1612
CCGAAGG8650.021.1613
CACCGAA8700.021.0393871
TTTTTTC651.2732926E-1020.97843221
TCTACCC250.003389387420.97843220
ATTTCTG2000.020.97843220
ATTTTCT303.9437655E-420.97843220
GTTTTTC456.388891E-720.9784320
GTAGATT354.621935E-520.97277616
Produced by FastQC (version 0.11.2)