FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_34.3510000005b546.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_34.3510000005b546.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54153
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC2820.5207467730319649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCTC404.6371224E-621.3042263
ACACGTC506.207847E-821.3042261
ACGTCTC506.207847E-821.3042263
CTCCTCT404.6371224E-621.3042262
CACGTCT506.207847E-821.3042262
ACTCCTC404.6371224E-621.3042261
ACCCGTC1000.021.3042261
TAGTCTC303.5081987E-421.3042263
ACTCATC751.8189894E-1221.3042261
CCCGTCT1000.021.3042262
ATTCGTC455.358361E-721.3042261
GCGTCTC1400.021.3042263
CTAGTCT303.5081987E-421.3042262
ACTAGTC303.5081987E-421.3042261
TCATCTC751.8189894E-1221.3042263
ACGCGTC1400.021.3042261
CGCGTCT1400.021.3042262
ACTCGTC48200.021.3042241
TCGTCTC48750.021.3042243
CTCGTCT48250.021.3042242
Produced by FastQC (version 0.11.2)