FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_33.3510000005b52c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_33.3510000005b52c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71981
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA2100.291743654575513No Hit
AACGGAATGGCTGCTGTCTCTTATACA1230.1708784262513719No Hit
AACGGAATGGCTCCTGTCTCTTATACA1180.16393214876147874No Hit
AACGGAATGGCTCTGTCTCTTATACAC980.13614703880190604No Hit
AACGGAATGGCTGTCTCTTATACACAT850.11808671732818382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGAA850.021.4501441
AACGCAA404.4037824E-621.4501421
AACGAAA607.8216544E-1021.4501421
ATCGGAA751.8189894E-1221.4501421
AACGGAA65800.021.4501421
ACGCAAT404.431118E-621.4348752
TCGGAAT751.8189894E-1221.4348752
CGAAATG607.894414E-1021.4348753
ACGAAAT607.894414E-1021.4348752
CGCAATG404.431118E-621.4348753
ACGGAAT65800.021.4348742
CGGAATG66550.021.4187723
TGGTTTT1400.020.92842318
TCCTTTT456.42327E-720.92842318
GTGTTTT1500.020.92842318
CAATGGC405.4540524E-620.9284235
CAGTTTT800.020.92842318
ATCTTTT751.8189894E-1220.92842318
AGCTTTT800.020.92842318
TCGATTT354.64678E-520.92842317
Produced by FastQC (version 0.11.2)