FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_32.3510000005b51f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_32.3510000005b51f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45204
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC2180.48225820723829754No Hit
GGATAGTTAGCCTGTCTCTTATACACA910.20130961861782143No Hit
GGATAGTTAGCCGCTGTCTCTTATACA500.11060968055924254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTT2200.021.4280872
GATCGTT1750.021.4280872
GGATCGT1750.021.4280871
GGTTAGT1300.021.4280871
GTTAGTT1300.021.4280872
GGCTAGT2200.021.4280871
ATCGTTA1750.021.4280873
TTAGTTA1300.021.4280873
ATAGTTA38650.021.4280853
GGATAGT38500.021.4002571
GATAGTT38450.021.4002232
CTAGTTA2250.020.9519063
TGTTGTT250.003395279920.93031116
AGCCCGC250.003395279920.9303119
AGCCCCT250.003395279920.9303119
AGCCCCG507.42275E-820.9303119
AGCCCCC507.42275E-820.9303119
AGCCTCT507.42275E-820.9303119
AGCCTCA250.003395279920.9303119
AGCCTAT558.749339E-920.9303119
Produced by FastQC (version 0.11.2)