FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_30.3510000005b4cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_30.3510000005b4cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49611
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC2480.4998891374896696No Hit
AGTGTGACGAACGGGGGGCTGTCTCTT520.10481546431235007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGACG44300.021.5420573
GGTGACG1150.021.5420573
AGTGTGA44250.021.5420571
GGGTGAC1150.021.5420572
TGAGACG607.239578E-1021.5420553
TGCGACG250.002875503921.5420553
GTGAGAC607.239578E-1021.5420552
AGTGCGA250.002875503921.5420551
AGTGAGA607.239578E-1021.5420551
GTGTGAC44200.021.5420552
GCGTGAC1100.021.5420532
AGTGACG556.3027983E-921.5420533
AGCGTGA1100.021.5420531
GGATTTT1350.020.912318
GAACGAG1550.020.91239
GAACTGG1750.020.91239
CGAACTC1750.020.91238
CGAACGT4350.020.91238
GGGGTTT1150.020.912317
AACGGGG2150.020.912310
Produced by FastQC (version 0.11.2)