FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_3.3510000005b205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_3.3510000005b205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195154
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC19621.0053598696414114No Hit
GAGGAACAGAGCTGTCTCTTATACACA3710.19010627504432398No Hit
GAGGAACAGAGTGGGGGCTGTCTCTTA2380.12195496889635876No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAAC303.527083E-421.3287261
AGAACAG303.527083E-421.3287263
AGAAACA303.527083E-421.3287262
AAGAACA303.527083E-421.3287262
AGGTACA1200.021.3287262
GAGGCAC13250.021.3287241
ATGAACA2150.021.3287242
AGGAACA170950.021.3287242
GATGAAC2150.021.3287241
AGGCACA13250.021.3287242
TGGAACA2450.021.3287242
GGAACAG173400.021.3287243
GAGGAAC170850.021.3162381
GGCACAG13300.021.248543
GTTTGTA303.9859518E-420.94619811
GTGTTTG303.9859518E-420.94619811
GTTTGGA303.9859518E-420.94619811
GTGCACA303.9859518E-420.94619811
CTCCTTT303.9859518E-420.94619817
ATGATTT303.9859518E-420.94619817
Produced by FastQC (version 0.11.2)