FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_26.3510000005b447.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_26.3510000005b447.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50140
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC6921.3801356202632629No Hit
ACTGTCTCTGTCTCTTATACACATCTC5491.0949341842840048No Hit
ACTGTCTGTCTCTTATACACATCTCCG880.17550857598723574No Hit
ACTGTCTGTGTCCCTGTCTCTTATACA660.13163143199042682No Hit
ACTGTCTGTGTCTGTCTCTTATACACA610.12165935380933388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTG505.9391823E-821.3853232
ATTGTCT505.9391823E-821.3853231
CAGTCTG303.413957E-421.385322
ACTGACT404.4749468E-621.385321
ACCGTCT455.148813E-721.385321
CCGTCTG455.148813E-721.385322
CTGACTG404.4749468E-621.385322
CTGTCTG45050.021.3615862
ACTGTCT46450.020.9939861
TGGTTTT1250.020.93712618
ACTGTGT405.380127E-620.9371265
GTGTCTC2400.020.9371268
GGGTTTT2400.020.93712618
GGCATTT250.003393961120.93712617
GTGTCGC4550.020.9371268
AGCTTTT601.036824E-920.93712618
GTGTCCT3650.020.9371268
GTGTCCC1600.020.9371268
GTGTCCA2100.020.9371268
GTGTCAT2200.020.9371268
Produced by FastQC (version 0.11.2)