FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_25.3510000005b42d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_25.3510000005b42d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64811
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC1650.25458641280029626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGTT1450.021.3252161
TCGGTTC800.021.3252162
CTTGCTT506.191658E-821.3252161
CGGTTCG800.021.3252163
CTGGTTC659.822543E-1121.3252142
CTTGATT659.822543E-1121.3252141
TGGTTCG60100.021.3074743
CTTGGTT58400.021.3069571
TTGGTTC58400.021.2886982
TTGTCTG405.3991116E-620.9467610
AACGTTT250.003395550720.9467617
TCCTTTT701.4551915E-1120.9467618
GTTGATG950.020.946769
TGTTGGG250.003395550720.9467611
TGCATTT354.6064117E-520.9467617
TGGTCGG250.003395550720.9467611
TGGTTGA250.003395550720.9467611
TTGTTAC250.003395550720.9467610
GTTGAGT1000.020.946769
GTTGAGC1450.020.946769
Produced by FastQC (version 0.11.2)