FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_23.3510000005b3e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_23.3510000005b3e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51319
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA3540.6898029969407042No Hit
ATCTGCTATCCTGTCTCTTATACACAT930.18121943139967653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTAT47350.021.3944243
ACTGCTA303.4056982E-421.3944232
ATCTCCT607.912604E-1021.3944231
CTCCTAT607.912604E-1021.3944233
AACTGCT303.4056982E-421.3944231
TATGCTA505.9195372E-821.3944232
ATCTACT950.021.3944231
TCTACTA950.021.3944232
TCTGCTA46250.021.3944232
GCTGCTA659.094947E-1121.3944232
ATGCTAT505.9195372E-821.3944233
TCTCCTA607.912604E-1021.3944232
AGCTGCT659.094947E-1121.3944231
TTTGCTA659.094947E-1121.3944232
CTACTAT950.021.3944233
TTGCTAT659.094947E-1121.3944233
ATCTGCT46300.021.3713171
GTTTGTT303.9443106E-420.93567516
CCGGATG303.9443106E-420.93567510
CCGGAGG601.0404619E-920.93567510
Produced by FastQC (version 0.11.2)