FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_20.3510000005b38b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_20.3510000005b38b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51126
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCCTACGTCTGTCTCTTATACAC4830.9447247975589719No Hit
AGTCTCCTACCTGTCTCTTATACACAT4760.9310331338262333No Hit
AGTCTCCTACGCTGTCTCTTATACACA1300.25427375503657634No Hit
AGTCTCCTACGTGGCTGTCTCTTATAC570.11148926182372962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCTA556.697519E-921.4328673
AGTCACC556.697519E-921.4328671
AGCCTCC950.021.4328651
ACTCCTA751.8189894E-1221.4328653
CCTCCTA950.021.4328653
TTCTCCT455.0435847E-721.4328652
GTCTCCT45350.021.4328652
GGCTCCT1050.021.4328652
AGGCTCC1050.021.4328651
TCTCCTA45800.021.4328653
GCTCCTA1050.021.4328653
AGACTCC751.8189894E-1221.4328651
AGTCCCC404.3921646E-621.4328651
ATTCTCC455.0435847E-721.4328651
AGTCTCC45350.021.4328651
GACTCCT751.8189894E-1221.4328652
TCCCCTA404.3921646E-621.4328653
GGCATTT558.822099E-920.92955217
ACGTTGG1100.020.9295529
ACGTCCT558.822099E-920.9295529
Produced by FastQC (version 0.11.2)