FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_19.3510000005b361.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_19.3510000005b361.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130402
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAGCGCATTCTGTCTCTTATACAC1720.13189981748746185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAGCC800.021.2732582
AGAGCCC800.021.2732583
AGCGCGC5000.021.2732583
CAGCGCG5000.021.2732582
CAGAGCA557.57791E-921.2732562
AGAGCAC557.57791E-921.2732563
AGAGCGC114200.021.2639453
TCAGAGC114050.021.2639311
CAGAGCG112750.021.2638232
CGAGCGC4800.021.0516623
CATTGTA2450.020.955149
CATTGCG2650.020.955149
GATATTT651.2732926E-1020.9551417
ATTAGGA651.2732926E-1020.9551410
GGTCTTT651.2732926E-1020.9551417
GATGTTT651.2732926E-1020.9551417
ATGGTTT651.2732926E-1020.9551417
CATTCTA1300.020.955149
GCGCGCA5000.020.955144
CATTTGT2650.020.955149
Produced by FastQC (version 0.11.2)