FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_15.3510000005b2f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_15.3510000005b2f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49154
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA3750.7629084103023152No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT2000.40688448549456807No Hit
TGGCATAGCTGAAGGGCTGTCTCTTAT870.1769947511901371No Hit
TGGCATAGCTGACGGGCTGTCTCTTAT720.14647841477804452No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC720.14647841477804452No Hit
TGGCATAGCTGAGGGCTGTCTCTTATA650.13223745778573462No Hit
TGGCATAGCTGAGGGGGCTGTCTCTTA620.12613419050331612No Hit
TGGCATAGCTGAGAGGCTGTCTCTTAT600.12206534564837043No Hit
TGGCATAGCTGAGGGCCTGTCTCTTAT590.12003092322089759No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT580.11799650079342475No Hit
TGGCATAGCTGAAGGGGCTGTCTCTTA570.1159620783659519No Hit
TGGCATAGCTGATGGGCTGTCTCTTAT540.10985881108353339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCATA659.094947E-1121.3929331
TGCATAG659.094947E-1121.3929332
TGGCATA42650.021.3929311
GTCATAG900.021.3929312
GGCATAG42550.021.3929312
TGTCATA900.021.3929311
TGGAATA505.910806E-821.3929311
GGAATAG505.910806E-821.3929312
TCATAGC900.021.3929313
TGGCCTA2900.021.3929311
GCATAGC43150.021.3929313
GCCTAGC2950.021.030343
GGCCTAG2950.021.030342
TGATGGT250.003394196520.93591110
TGATGGC405.3793865E-620.93591110
TGATGGA250.003394196520.93591110
GATGGGG250.003394196520.93591111
TGGTTTT950.020.93591118
TGATGAG354.5956152E-520.93591110
GAGGCGT405.3793865E-620.93591111
Produced by FastQC (version 0.11.2)