FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_14.3510000005b2dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_14.3510000005b2dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46780
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA4160.8892689183411715No Hit
ATGCTCTGTTGCGGGGCTGTCTCTTAT1220.26079521162890124No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC1100.235143223599829No Hit
ATGCTCTGTTGCGCCTGTCTCTTATAC670.14322359982898675No Hit
ATGCTCTGTTGCCCCTGTCTCTTATAC590.12612227447627192No Hit
ATGCTCTGTTGCGGGCCTGTCTCTTAT570.1218469431380932No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC550.1175716117999145No Hit
ATGCTCTGTTGCGGGGGCTGTCTCTTA500.10688328345446774No Hit
ATGCTCTGTTGCGGGGGGGGTTTTTTT470.10047028644719964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTCTG608.3491614E-1021.293482
ATGCTCT42150.021.293481
ATTCTCT608.3491614E-1021.293481
ACGCTCT404.634594E-621.2934781
ATGATCT455.3514486E-721.2934781
TGATCTG455.3514486E-721.2934782
ATGCCCT751.8189894E-1221.2934781
TGCTCTG42200.021.2682482
GCTCTGT43350.021.2197973
AGGGGTT303.9165592E-420.95187416
TCCTTTT303.9165592E-420.95187418
TTGCATG558.6656655E-920.9518749
TTGCATA303.9165592E-420.9518749
TTGCACG601.022272E-920.9518749
TTGCACA303.9165592E-420.9518749
TTGCAAC303.9165592E-420.9518749
TGCGCTC303.9165592E-420.95187410
TGCGCTA303.9165592E-420.95187410
TGCGTGT303.9165592E-420.95187410
TGCGTCG303.9165592E-420.95187410
Produced by FastQC (version 0.11.2)